Fig. 2

Differentially expressed lncRNAs in iPSC-ECs treated with e-cig. A Volcano plot of differentially expressed lncRNAs is shown, where red and blue dots represent lncRNAs that are up- and downregulated, respectively, with at least a fold change of 2 and p value < 0.05. B Hierarchical clustering analysis showing differentially expressed lncRNAs between non-treated (control) and treated groups with e-cig (6.5 TPE EAE). C PCA of lncRNA expression profiles is shown, with treated and untreated samples connected for each subject. A qPCR validation of D up- and E downregulated lncRNAs following EAE treatment (6.5 TPE). Data were obtained using iPSC-ECs from four healthy donors, and the assays were repeated three times. Data are represented as mean ± SD. * and ** indicate p < 0.05 and p < 0.001, respectively