Fig. 4

PINK1 deficiency impairs mitochondrial calcium uptake. a Experimental protocol for live cell imaging of preosteoblasts from mouse embryo calvaria. b Fluorescent time-lapse images of mito-LAR-GECO1.2-expressing preosteoblasts at the indicated times post-ionomycin treatment (500 nM). c Quantified intensity of Mito-R-GECO1 fluorescence in each indicated preosteoblast. Data from three representative cells have been shown for each treatment. Trajectories for each cell have been represented in a different color. Fluorescence intensity, quantified as average pixel intensity per cell, using the same confocal imaging system. Scale bar = 20 μm