Fig. 2
Neuroepithelial derivation and characterization. a Schematic timeline and images of NE derivation. During the first 8 days, hIPS are induced to form EBs in suspension with hIPS media without FGF during the first 4 days and then switch to NIM. At day 8 the EBs are plated to form neural rosettes (middle image). At day 17, rosettes are manually selected and further cultured to obtain mature NE until the desire point of maturation. b Immunocytofluorescence of NE cultures showing immature (GFAP, DCX, LHX2, OTX2, PAX6) and mature neuronal markers (BIII Tub, MAP2, Vglut-1, GAD67). c NE markers quantification at 4 (Map2p<0.0038, BIII tub, GFAPp<0.0002, Otx2 and Pax6;) and 10 (Map2, BIII tub, DCXp<0.0003, Gad67, GFAP, Vglut-1p<0.019, Lhx2, Otx2 and PAx6) weeks of differentiation. Data was analyzed by Two-way ANOVA with Bonferroni Test