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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: A novel mutation of SATB2 inhibits odontogenesis of human dental pulp stem cells through Wnt/β-catenin signaling pathway

Fig. 4

SATB2 regulated osteo/odontogenic differentiation of hDPSCs through Wnt/β-catenin signaling pathway. a Quantitative RT-PCR showed that SATB2 knockdown remarkably up-regulated DKK1 expression. b Western blot analysis showed that SATB2 knockdown down-regulated active β-catenin expression. c The hDPSCs transfected with wild-type SATB2 showed lower level of DKK1 compared with vehicle, while mutant SATB2 transfection expressed higher level of DKK1 compared with the ones transfected with wild-type SATB2, as assessed by q-PCR analysis. d Wild-type SATB2 transfection induced higher level of active β-catenin compared with vehicle. The expression of active β-catenin was lower in the hDPSCs transfected with mutant SATB2 in comparison to those transfected with wild-type SATB2. e Alizarin Red S staining showed that XAV939 decreased osteo/odontogenesis of hDPSCs and the increased mineralized nodule formation induced by SATB2 transfection was blocked by XAV939. f, g Quantitative RT-PCR showed that XAV939 decreased ALP and RUNX2 expression in hDPSCs. The increased expression of ALP and RUNX2 induced by wild-type SATB2 transfection was impaired after the inhibition of Wnt/β-catenin signaling pathway. Scale bar 200 μm, *P < 0.05, **P < 0.01, ***P < 0.001. Data are expressed as the means + SD. Each experiment was repeated three times with n ≥ 3 samples per group. si NC: negative control siRNA transfection, si SATB2: SATB2 siRNA transfection, Vector: vector transfection, WT SATB2: wild-type SATB2 transfection, MU SATB2: mutant SATB2 transfection, Vector + XAV939: vector transfection and XAV939 added, WT + XAV939: wild-type SATB2 transfection and XAV939 added

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