Fig. 3

Cell-scaffold interaction in vitro. A Live/dead staining of hPDLSCs after 7 days of culture on random and aligned silk scaffolds. Viable cells were stained by calcein-AM (green) and dead cells were stained by PI (red). B Cell seeded scaffolds were observed under SEM. C Proliferation of hPDLSCs cultured on silk scaffolds for 3 and 7 days, which was measured by CCK-8. D Human PDLSCs were seeded on scaffolds and cultured in the tenogenic differentiation medium for one week. The expression of COL I was evaluated by immunofluorescence staining. **p < 0.01