Fig. 4

Animal experiment and tracing of implanted cells. A Schematic illustration of animal model and grouping, as well as the gross morphology of repaired rat Achilles tendon. R group, random silk scaffold alone. A group, aligned silk scaffold alone. AC group, hPDLSCs seeded aligned silk scaffold. B Immunohistochemistry with anti-human nuclei monoclonal antibody. Red arrows indicate the transplanted human cells, nuclei of which were dyed by both haematoxylin and DAB. Yellow arrows indicate the nuclei of rat cells dyed by haematoxylin only. C Negative control of immunohistochemistry