Fig. 1

BMSC transplantation rescues T2D-associated NAFLD. A Experimental design for establishing T2D model and assessing the BMSC treatment on T2D-associated NAFLD. Six-week C57BL/6 J mice were fed with HFD throughout the trial. Two BMSC injections were performed at 32nd and 34th week. Additionally, two vehicle injections (normal saline) were set as control. B The dynamic monitoring of mice after BMSC or vehicle administration (started at 34th week). N = 10 animals per group. C Representative images of ND mice and T2D-associated NAFLD mice administered with vehicle or BMSCs. The images were photographed at 45th week. D GTT and E ITT analyses were carried out to evaluate the sensitivity of mice at 45th week to glucose and insulin. N = 6 animals per group. F Serum biochemical assay of mice at 45th week. Sera of mice at 45th week were isolated to detect ALT, AST, TG, and TCHO levels. N = 6 animals per group. G Representative images of gross liver and assays for HE and oil red O-stained liver sections. H Image J software was utilized to assay steatosis degree of liver. N = 7 animals per group. I Homogenized liver tissues were subjected to TG detection. N = 6 animals per group. All statistical data are represented as means ± s. *P < 0.05; **P < 0.01; ***P < 0.001. All imaging was performed and analyzed in a blinded fashion