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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Bone marrow derived-mesenchymal stem cell improves diabetes-associated fatty liver via mitochondria transformation in mice

Fig. 3

BMSC intervention directly enhances the mitochondrial activity in vitro. A The establishment of HepG2-mito-GFP cell lines. The mitochondria were stained with MitoTracker Red. Scare bar represents 25 μm. B Trial design for establishing steatotic cell model and assessing the BMSC intervention effect: well-growing HepG2-mito-GFP cells were fed with FFA medium for 36 h, and then BMSCs were added for 48 h co-cultivation, followed by puromycin screening. Cells fed with complete medium were set as control. C Oil Red O stain detection of treated cells in B and the quantification of Oil Red O staining area. Three independent replicates were performed. D Representative TEM imaging of treated cells in B and the quantification of average mitochondrial number in the field at 500 nm. Big droplets are labeled with asterisk. Twenty-five fields per group were taken into consideration. E Total ROS level was detected via FCM and quantitative analysis at 24 h and 48 h. Three independent replicates were performed. F WB assay of target protein level. The relative expression level was determined by densitometry and normalized to β-actin levels. All statistical data are represented as means ± s. *P < 0.05; **P < 0.01; ***P < 0.001

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