Fig. 6

Mitochondrial transfer dominates the rescue of steatotic cells over paracrine signaling. Cells were processed as the description in Fig. 5F. A Representative micrographs of Oil Red O-stained cells and the quantification of stained area. B TEM imaging of differently treated cells, and the quantification of average mitochondrial number at 500 nm. Twenty-five fields per group were taken into consideration. C OCR assay of differently treated cells. D Relative OCR comparison in terms of basal, maximal, and spare respiratory levels. E Detection of relative ATP, MMP, and ROS levels in differently treated cells. ΒΜSCs-group and BMSCs-0.22 μm-group indicate BMSCs were planted at the upper chamber with 0.4 μm or 0.22 μm filter membrane, respectively. HepG2-group means HepG2 cells were planted at the upper chamber with 0.4 μm filter membrane. All trials above were performed with three independent replicates, and the statistical data are represented as means ± s. *P < 0.05; **P < 0.01; ***P < 0.001