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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Role of PD-L1 in licensing immunoregulatory function of dental pulp mesenchymal stem cells

Fig. 3

Activation of PD1/PD-L1 pathway in MSCs. A Western blot analyses of PD-L1 expression in DPSCs, AFSCs and BM-MSCs cultured alone and after co-culture with aPBMCs, either in direct co-culture conditions and transwell culture system. Histograms show that PD-L1 expression was statistically significant higher in MSCs after both direct co-culture and transwell culture conditions with aPBMCs. ***P < 0.001, *P < 0.05 versus MSCs cultured alone. Data are represented as mean ± SD and statistical analysis of differences between MSCs cultured alone and MSCs after co-culture was carried out by unpaired Student t test. B–E Human PD1 and PD-L1 proteins immunoprecipitation. B PD1 protein was immunoprecipitated and revealed with anti-PD1 antibody. C PD-L1 protein was immunoprecipitated and revealed with anti-PD-L1 antibody. D PD-L1 protein was immunoprecipitated with anti-PD-L1 antibody and revealed with anti-PD1 antibody. E PD1 protein was immunoprecipitated with anti-PD1 antibody and revealed with anti-PD-L1 antibody. CD95 antibody was used as negative control

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