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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Role of PD-L1 in licensing immunoregulatory function of dental pulp mesenchymal stem cells

Fig. 4

Evaluation of PD1/PD-L1 pathway and inflammatory cytokines expression in aPBMCs after DPSCs co-culture. A Expression of PD1 and PD-L1 was evaluated by flow cytometry on aPBMCs cultured alone and co-cultured with DPSCs with or without anti-PD-L1 blocking antibody. Median fluorescence intensity obtained by PD1 and PD-L1 staining minus median fluorescence intensity in FITC and PECy7 channels of FMO controls is shown in CD4+ and CD4 gated T lymphocytes. Data are expressed as mean ± SD and analyzed by one-way ANOVA followed by Tukey post-hoc test. *P < 0.05 aPBMCs + PD-L1 inib, aPBMCs after DPSCs co-culture + PD-L1 inib vs. aPBMCs; §P < 0.05, §§P < 0.01 aPBMCs after DPSCs co-culture + PD-L1 inib vs. aPBMCs after DPSCs co-culture. B The expression of IFNγ, TNFα, IL-2, IL-6, IL-10, CCL5 and CXCL10 was evaluated through Real Time PCR analyses on rPBMCs and aPBMCs alone, aPBMCs after DPSCs co-culture with and without PD-L1 selective inhibitor. Data are expressed as mean ± SD and analyzed by one-way ANOVA followed by Newman-Keuls post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001 vs. rPBMCs; §§P < 0.01, §§§P < 0.001 vs. aPBMCs; °°°P < 0.001 vs. aPBMCs after DPSCs co-culture. C The expression of caspase 3 and PCNA on aPBMCs cultured alone and after DPSCs co-culture with and without PD-L1 inib was evaluated by Western blot analyses. SC consisted in cleaved-caspase 3 positive control. *P < 0.05, **P < 0.01 §P < 0.05 vs. aPBMCs alone

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