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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Culture and characterization of various porcine integumentary-connective tissue-derived mesenchymal stromal cells to facilitate tissue adhesion to percutaneous metal implants

Fig. 5

Assessment of in vitro adipogenic differentiation of pMSCs. a Histological staining (Oil Red O) of the nine pMSCs cultured in adipogenic media after 21–28 days in culture (50 × magnification). b Quantification of intracellular lipid droplet formation by measuring absorbance of solubilized Oil Red O at 500 nm. cd Fold change in gene expression of the adipogenic transporter AP2 (c) and receptor PPAR-ɣ (d) in pMSCs cultured in adipogenic media (AM) versus growth media (GM). β-Actin was used for normalization of gene expression. Data presented as mean ± SEM (n = 3; *p < 0.05)

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