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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Extracellular vesicles from adipose-derived stem cells promote microglia M2 polarization and neurological recovery in a mouse model of transient middle cerebral artery occlusion

Fig. 1

Characterization of ADSCs and ADSC-derived EVs. A Flow cytometric analysis of ADSCs surface markers CD29 and CD44, and negative markers CD34 and CD45. Black curve: Unstained ADSCs; Red curve: ADSCs stained by fluorescent labeled primary antibodies. B Representative TEM image of ADSC-EVs. Scale bar = 500 nm. The enlarged picture showed the clear structure of ADSC-EVs. Scale bar = 100 nm. C Size distribution of ADSC-EVs measured by NTA. D Expressions of the EV markers CD63, TSG101, and β-actin confirmed by Western blot. ADSC-EVs lysate was loaded into the left lane and ADSCs lysate was loaded into the right lane

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Stem Cell Research & Therapy

ISSN: 1757-6512