Fig. 3

ADSC-EVs attenuated white matter injury and promoted angiogenesis in tMCAO mice. A Immunofluorescence staining of MBP at 14 days after tMCAO in the PBS and ADSC-EVs groups. Scale bar = 1000 μm. The enlarged pictures of white square revealed MBP staining in the striatum of the ipsilateral and the contralateral hemisphere. Scale bar = 200 μm. B Quantitative analyses of the ratio of mean MBP area in ipsilateral to contralateral striatum. C Quantitative analyses of the ratio of mean MBP intensity in ipsilateral to contralateral striatum. n = 3 in the sham group, n = 4 in the PBS and ADSC-EVs groups. D Immunofluorescence staining of CD31⁺ microvessels in the ipsilateral hemisphere of mice brain after tMCAO in the PBS and ADSC-EVs groups. The images of the contralateral hemisphere were presented as control. White star indicated the core area of infarction and the white dashed line represented the boundary between the ischemic core area and peripheral area. Scale bar = 50 μm. E Mean integrated density of CD31⁺ microvessels in the perifocal region at 14 days after tMCAO. F Quantifications of CD31⁺ microvessels numbers in the perifocal region at 14 days after tMCAO. n = 4 in the PBS and ADSC-EVs groups. G Immunofluorescence staining of Ki67⁺/CD31⁺ newly proliferated endothelial cells in the ipsilateral hemisphere of mice brain after tMCAO in the PBS and ADSC-EVs groups. The images of the contralateral hemisphere were presented as control. White arrows indicated Ki67⁺/CD31⁺ cells. Scale bar = 20 μm. H The enlarged picture showed the Ki67⁺/CD31⁺ cells. I Quantifications of Ki67⁺/CD31⁺ cell numbers in the perifocal region at 14 days after tMCAO. n = 4 in the PBS and ADSC-EVs groups. Scale bar = 20 μm. Data were mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001