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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Extracellular vesicles from adipose-derived stem cells promote microglia M2 polarization and neurological recovery in a mouse model of transient middle cerebral artery occlusion

Fig. 7

ADSC-EVs inhibited LPS or OGD induced up-regulation of STAT1 and PTEN. A The relationship between 14 up-regulated miRNAs and proteins related to microglia polarization were analyzed by IPA, and STAT1 and PTEN were highlighted as the targets of these miRNAs. B qPCR revealed that ADSC-EVs administration did not significantly change STAT1 and PTEN expression in primary microglia under normal condition. C ADSC-EVs pretreatment attenuated LPS induced up-regulation of STAT1 and PTEN. D ADSC-EVs post-treatment significantly dampened the OGD-induced upregulation of STAT1 and PTEN. E qPCR confirmed that STAT1 expression was down-regulated after the transfection of miR-93-3p mimics, miR-128-3p mimics, miR-144-3p mimics, miR-146a-5p mimics, and miR-223-3p mimics compared with the negative control in BV2 microglia. The expression of PTEN was down-regulated after the transfection of miR-106b-5p mimics, miR-200a-3p mimics, miR-223-3p mimics, and miR-363-3p mimics. n = 3 per group. Data were mean ± SEM of three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001

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