Fig. 1

Viability, proliferation and ROS expression in CD34⁺ cells after co-culture with MSC WT or HIF-MSC. Apoptosis assays in CD34⁺ cells previously co-cultured with MSC WT or HIF-MSC during 72 h. CD34⁺ cells were incubated with Annexin V, 7-AAD and CD34, and the expression of different cell surface markers was analyzed by flow cytometry. Cells were classified as viable (Annexin V⁻/7-AAD⁻), early apoptotic (Annexin V⁺/7-AAD⁻), late apoptotic (Annexin V⁺/7-AAD⁺) or dead (Annexin V⁻/7-AAD⁺). Data expressed as mean of the percentage of cells in the different conditions (A). Cell cycle profiling of CD34⁺ cells after 72 h of culture in each condition analyzed by flow cytometry. Data are represented as mean of the percentage of cells in each phase (B). Intracellular reactive oxygen species (ROS) expression of each population represented as median of fluorescence intensity (C). Ten experiments were done for each group. p:n.s means p > 0.05