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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Co-administration of human MSC overexpressing HIF-1α increases human CD34+ cell engraftment in vivo

Fig. 2

Expression of surface proteins involved in hematopoiesis and engraftment in CD34+ cells and clonogenic capacity. Median fluorescence intensity of different surface proteins involved in hematopoiesis maintenance and engraftment as CD34, CXCR4, ITGA-4 and c-KIT was evaluated by flow cytometry. Samples were acquired on a FACS Canto flow cytometer (A). Total CFU-GM from CD34+ cells were scored after 14 days in methylcellulose medium. CD34+ cells were cultured with MSC WT or HIF -MSC for 72 h, and then, 1,500 cells were seeded into methylcellulose medium (B). Data are represented as mean of 10 experiments for each group. p:n.s means p > 0.05

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