Fig. 3

Analysis of human hematopoietic engraftment in vivo. Human hematopoietic engraftment was evaluated by flow cytometry after xenotrasplantation in NOD/SCID mice. The percentage of human CD45⁺ cells (donor chimerism) was analyzed in total bone marrow samples from both femurs, right (MSC injected site) and left (contralateral) 4 weeks after transplantation. Mice were transplanted intravenously with CD34⁺ cells alone or co-transplanted with MSC WT or HIF-MSC, that were administered in the right femur (A). Percentage of human hematopoietic subpopulations (CD34, CD14, CD13 and CD19) in the injected femur after 4 weeks was also analyzed (B). Data are represented as mean of 8 experiments for mice transplanted with CD34⁺ cells alone, 13 experiments for mice co-transplanted with CD34⁺ cells and WT MSC and 15 experiments for mice transplanted with CD34⁺ cells and HIF-MSC. p:n.s means p > 0.05