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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: DZNep promotes mouse bone defect healing via enhancing both osteogenesis and osteoclastogenesis

Fig. 3

DZNep promoted osteoclast formation by inducing the p-P65 nuclear translocation. A NFATc1 and c-Fos expression levels in BMMs treated with or without 25 nM of DZNep for 0, 1, 3, and 5 days. B The gray levels of NFATc1 and c-Fos were quantified and normalized to β-actin using ImageJ. C NFATc1 and c-Fos expression levels in BMMs stimulated with M-CSF (30 ng/mL), and RANKL (50 ng/mL) for 5 days in presence of a various concentration of DZNep. D The gray levels of NFATc1 and c-Fos were quantified and normalized to β-actin using ImageJ. E BMMs were pretreated with 25 nM of DZNep for 3 h before the addition of RANKL for 30 min. Nuclear translocation of p-P65 was analyzed using western blotting. F RAW264.7 cells were pretreated with 25 nM DZNep for 2 h, and then RANKL was added for 1 h. Nuclear translocation of p-P65 was then visualized by immunofluorescence. Scale bar = 20 nM (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). Data are expressed as median and interquartile range, n = 3

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