Fig. 4

DZNep promoted the p-P65 nuclear by facilitating the phosphorylation of IKKα/β, and the degradation of IκBα instead of the MAPK pathway. A RAW264.7 cells were pretreated with 25 nM DZNep for 2 h before the stimulation with RANKL for specific times, and the levels of NF-κB signaling pathway proteins were determined. B The gray levels of p-P65 were quantified and normalized to β-actin using ImageJ. C The gray levels of p-IκBa were quantified and normalized to β-actin using ImageJ. D RAW264.7 cells were pretreated with 25 nM DZNep for 2 h before the RANKL was added on different time points, and the expression of MAPK signaling pathway proteins was explored. E The gray levels of p-ERK were quantified and normalized to β-actin using ImageJ (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). Data are expressed as median and interquartile range, n = 3