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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: DZNep promotes mouse bone defect healing via enhancing both osteogenesis and osteoclastogenesis

Fig. 8

DZNep showed the therapeutic effect on bone defect in vivo. A The defect tibias of mice treated with or without DZNep were scanned with animal X-ray, and two-dimensional images are presented. B The fixed tibias of bone defect mice divided into two groups and treated for 2 weeks were analyzed by mCT, and three-dimensional reconstructed images are presented. Scale bar = 50 μm (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). C Microstructural indices include bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular thickness(Tb.Th), and trabecular separation (Tb.Sp) obtained by analyzing the two mouse groups are presented. D H&E (magnification × 4 and × 10) staining, respectively, of the defect tibia sections. Scale bar = 25 μm and 10 μm. E TRAP (magnification × 4 and × 10) staining, respectively, of the defect tibia sections. Scale bar = 25 μm and 5 μm. F The number of TRAP-positive cells and OcS/BS%. G Immunofluorescence staining, respectively, of Ocn in the defect tibiae sections. Scale bar = 25 μm. H Schematic representation of the molecular mechanism of the article (created with BioRender.com). Data are expressed as median and interquartile range, n = 3–6

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