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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Multilineage-differentiating stress-enduring cells alleviate atopic dermatitis-associated behaviors in mice

Fig. 1

Pruritus in the back skin caused by 2,4-dinitrofluorobenzene (DNFB) was attenuated by Muse cells. A DNFB model time course. Mice were recorded to establish a baseline the day before 50 μl of 0.5% DNFB was applied to the abdominal skin. Five days later, 30 μl of 0.25% DNFB was administered to the skin of the nape of the neck and was further applied on the 3rd, 5th, and 7th days. The day after the last administration, Muse cells or PBS was injected into the back skin of the mice. The black arrows in this diagram indicate the time points at which scratching behavior were recorded. B Muse cell suspension culture (left) and adherence culture (right). Scale bar = 100 μm. C The bar graph shows the scratching behavior of mice administered DNFB with and without subcutaneous injection of Muse cells. On day 1–5, the number of scratches in the Muse cell-treated group was significantly reduced compared with that in the PBS group. Muse cell treatment quickly reversed the itching and scratching times. One-way or Two-way ANOVA followed by the Bonferroni test; #p < 0.05 versus BL; ***p < 0.001, **p < 0.01, *p < 0.05 versus vehicle group; n = 5 mice per group. The data are presented as the mean ± s.e.m. The green arrows indicate the time of DNFB painting and the red arrow indicates the time point of Muse cells or PBS injection. D The cultured suspension of dyed Muse cells was injected subcutaneously into the damaged skin. Day 1, 3, 5, 7, 9 and 11 post injection, the mice were sacrificed and skin tissues were collected. The number of Dil-labeled Muse cells (red) gradually declined, and only a few cells remained on day 11 after injection. Scale bar = 100 μm

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