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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Multilineage-differentiating stress-enduring cells alleviate atopic dermatitis-associated behaviors in mice

Fig. 5

The protective effects of Muse cell supernatant on LPS-induced HaCaT cells. AC The mRNA expression of IL-17α, IL-6 and IL-33 in HaCaT cells was measured by real-time PCR. Each inflammatory factor was subjected to three independent repeated analyses, and HaCaT cells without Muse cell supernatant were used as the vehicle group. The results were normalized to Gapdh. The results showed that under inflammatory infection conditions, the increase in proinflammatory cytokines was powerfully inhibited by pretreatment with Muse cell supernatant. Two-tailed Student’s t-test; ***p < 0.001, **p < 0.01 versus vehicle group; n = 3–4 cultures per group. The data are presented as the mean ± s.e.m

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