Fig. 3
From: Induction of meiosis by embryonic gonadal somatic cells differentiated from pluripotent stem cells
Induction of E12.5_GSCLCs by small molecules. A Schematic illustration of in vitro chemical induction strategy. B qPCR detection of Gata4 and Foxl2 expression in GSCLCs induced by treatment with different small-molecule compounds. Bars = Mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. C qPCR detection of Gata4 and Foxl2 expression in GSCLCs induced by AM580 and V580. Bars = Mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. D Immunofluorescence staining of Gata4 (green) and Foxl2 (red) in GSCLCs induced by V580. Scale bar = 20 μm. E Protein levels of pluripotency markers (Nanog and Oct4) and E12.5_GSCs markers (Gata4 and Foxl2) in GSCLCs induced by V580 were determined via western blot analysis. β-actin served as a loading control. F Heatmap highlighting DEGs compared with E12.5_GSCs, determined using RNA-seq. G PCA shows that V580_D6 GSCLCs and E12.5_GSCs were closer with regard to the overall transcriptome