Fig. 5

Cell types in the induced GSCLCs revealed by single-cell RNA-seq analysis. A Left: U-map showing the distribution of E12.5_GSCs. Middle: Marker genes are indicated by color; expression gradually increases from gray to blue. Right: Proportion of each cell cluster at E12.5. B Left: U-map showing the distribution of V580_D6 GSCLCs. Middle: Marker genes are indicated by color; expression gradually increases with from gray to blue. Right: Proportion of each cell cluster among V580_D6 GSCLCs. C Left: U-map showing the distribution of CD63⁺_GSCLCs Middle: Marker genes are shown by the color; expression gradually increases from gray to blue. Right: Proportion of each cell cluster among CD63⁺_GSCLCs. D Percentage of PGCs that can initiate meiosis in the CD63⁺ group was slightly upregulated. E Percentage of synaptonemal complexes in the CD63⁺ group based on pachytene spread (n = 40) was significantly upregulated. Bars = Mean ± SEM (n = 10). *P < 0.05; **P < 0.01; ***P < 0.001