Fig. 7

Vasa⁺ cells can be detected in CD63⁺_rOvaries cultured for 10 days. A GO and KEGG analysis of CD63⁺_GSCLCs. B Schematic of the IVDi culture. C Morphology of rOvaries in the culture system on day 2 and day 10. Scale bar = 50 μm. Pure PGC is scattered and nearly disappears on day 10. Scale bar = 50 μm. D Proportion of Vasa⁺ cells in different groups. Vasa⁺ cells can be detected in Gonad_ and CD63⁺_ rOvaries cultured for 10 days in vitro, but no such cells were observed in MEF_ and P_6w_ rOvaries. Bars = Mean ± SEM (n = 10). *P < 0.05; **P < 0.01; ***P < 0.001. E Distribution of Vasa, Foxl2, and Gata4 in E12.5_rOvaries. Scale bar = 20 μm. F Distribution of Vasa, Foxl2, and Gata4 in MEF_rOvaries. Scale bar = 20 μm. G Distribution of Vasa, Foxl2, and Gata4 in CD63⁺_rOvaries, GFP: CD63⁺_GSCLCs. Scale bar = 20 μm. H Distribution of Vasa, Foxl2, and Gata4 in P_6w_rOvaries. Scale bar = 20 μm