Fig. 1From: Corneal epithelial differentiation of human pluripotent stem cells generates ABCB5+ and ∆Np63α+ cells with limbal cell characteristics and high wound healing capacitySchematic presentation of the study design. Human pluripotent stem cells (hPSCs) were differentiated towards limbal stem cells using the method described in Hongisto et al. [18], and the resulting populations were compared with tissue-derived limbal stem cells in various time points for their limbal stem cell and immunogenicity related marker expressions, using immunofluorescence and flow cytometry. Cryopreserved hPSC-derived limbal stem cells were additionally analyzed for their wound healing potential via calcium imaging and scratch assayBack to article page