Fig. 4
Expression of human leukocyte antigen (HLA) class I and class II proteins. a Flow cytometry (FC) results of HLA-A,B,C (class I) and b HLA-DP,DR,DQ (class II) expression in day (d) 10–11 (n = 6), d24–25 (nHLA-A,B,C = 6, nHLA-DR,DP,DQ = 5) and passage 1 (p1, n = 3) hPSC-LSCs populations, and in p1 human LSCs (n = 1). For statistical analysis, paired t-test was used for comparison of day 10–11 and day 24–25 hPSC-LSC populations, and Mann–Whitney U-test was used for comparison of day 24–25 and p1 hPSC-LSC populations. c Representative FC histograms showing unstained (tinted black) controls in overlays with HLA-A,B,C- or HLA-DR,DP,DQ-stained populations. Representative hPSC-LSC data shown for line Regea08/017