Fig. 5
Calcium signaling properties. a Expression of purinergic receptor P2Y2 in p1 hPSC-LSCs. Scale bar, 20 μm. b Representative pseudocolored image time series of ATP-induced Ca2+ response in p1 hPSC-LSCs, visualized with Fluo-4 Ca2+ indicator. c Representative single cell selections from one parallel sample and corresponding curve cluster showing relative intensities as a function of time for each analyzed cell in a sample. Typical response (based on relative intensity peak during the ATP stimulation) is highlighted in black, strongest response in pink and weakest response in blue. d Representative regions of interest (ROIs) from one parallel sample and resulting kymograph from one representative ROI, with example curves of single non-oscillating and oscillating p1 hPSC-LS cells. Asterix marks the primary ATP-induced calcium peak. All data for hPSC-LSC line Regea08/017