Table 2 Antibodies used in flow cytometry. Manufacturer recommendations in brackets
Antibody | Conjugation | Amount | Manufacturer | Catalog #No |
|---|---|---|---|---|
Ms ABCB5 mAb, clone 3C2–1D12 | N/A | 5 μg/mL | Frank Lab* | – |
Donkey anti-Mouse IgG (H + L) secondary ab | AlexaFluor 647 | 2.5 μg/mL | Thermo Fisher Scientific | # A31571 |
Ms IgG1 isotype control, clone MOPC-21 | AlexaFluor 647 | 5 μg/mL | Thermo Fisher Scientific | # MA5-18168 |
Ms anti-Human ABCG2 mAb, clone 5D3 | APC | 1.2 (2) μg/mL | BD Biosciences | # 561451 |
Ms IgG2b, k isotype control, clone 27–35 | APC | 20 (20) μL | BD Biosciences | # 555745 |
Ms anti-Human HLA-A,B,C, clone G46-2.6 | APC | 16 (20) μL | BD Biosciences | # 562006 |
Ms anti-Human HLA-DR,DP,DQ, clone Tu39 | FITC | 18 (20) μL | BD Biosciences | # 562008 |