Fig. 1

Emilin-2 distribution in BM of adult mice. A–I Representative immunofluorescence for Emilin-2 (red) and Collagen IV (gray) in BM of femur of 1-month-old (A–C), 3-month-old (D–F) and 6-month-old (G–I) wild-type mice. Asterisks in panel I indicate sinusoids and vessels. J–L Representative immunofluorescence for Emilin-2 (green) and Collagen IV (gray) in BM of tibia of 6-month-old wild-type mice. Asterisks in panel L indicate sinusoids and vessels. M Whole-mount immunofluorescence staining for Emilin-2 (green) in BM of femur of 6-month-old wild-type mice. BM and compact bone areas are indicated. N, O Representative immunofluorescence for Emilin-2 (green) and Collagen IV (gray) in BM of femur of 6-month-old Emilin2 null mice. In panels C, F, I, M, O, nuclei were stained with Hoechst (blue). Scale bar, 100 μm. Col IV, Collagen IV. See also Additional file 1: Fig. S1