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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Emilin-2 is a component of bone marrow extracellular matrix regulating mesenchymal stem cell differentiation and hematopoietic progenitors

Fig. 4

Frequency of in vivo HSC and LT-HSC hematopoietic progenitors in adult wild-type and Emilin-2 null mice. BM were isolated from wild-type and Emilin2/ mice of 3,6 and 10 months of age and subjected to flow cytometry with different antibodies. A Representative flow cytometry analysis of HSC and LT-HSC in BM of 10-month-old mice, based on the identification of lineage-negative (Lin) cells and of Sca1, c-Kit, CD150 and CD48 markers. Live cells, identified with side scatter (SSC; granularity) and forward scatter (FSC; cell size) parameters (plot i), negative for Linmarker (plot ii), were selected and analyzed for Sca1 and c-Kit signal (plot iii). Double positive Sca1+/c-Kit+ events were identified as HSC (squared areas of plot iii) and subjected to further analysis based on CD150 and CD48 signals to identify LT-HSC (Cd150+/CD48; squared area of plot iv). B Percentages of HSC, calculated on total live cells and on Lin cells, n BM of 3-month-old (wild type, n = 4;Emilin2/, n = 4), 6-month-old (wild-type, n = 10; Emilin2/, n = 6) and 10-month-old (wild-type, n = 7; Emilin2/, n = 9) mice. C Percentage of LT-HSC, calculated on either total live cells, Lin cells and HSC cells, in BM of 10-month-old (wild-type, n = 3; Emilin2/, n = 4) mice

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