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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: IL-6 coaxes cellular dedifferentiation as a pro-regenerative intermediate that contributes to pericardial ADSC-induced cardiac repair

Fig. 1

Isolation and purification of eGFP:WT1 cells from pericardial adipose tissue. A Expression of an injury-responsive element, Wilms Tumor factor 1 (WT1), which was almost undetectable in the uninjured heart (Sham), was found to be induced after myocardial infarction (MI, 7 days post in injury, dpi) in both epicardial layer (epi) and pericardial tissue (peri). B Schematic drawing shows the crossbreeding of mouse strain in which a reporter gene (enhanced green fluorescent protein, eGFP) was driven by WT1 expression (left) and irreversibly labeled the WT1 expressing cells in epicardial and pericardial tissue (right). C EGFP:WT1 cells from the pericardial adipose stromal tissue (ADSCgfp+) was isolated and cultured (left), and segregated form the non-WT cells (ADSCgfp−) by fluorescence-activated cell sorting (FACS, right). D Both ADSCgfp− and ADSCgfp+ cells were cultured and passaged with relatively high purity (> 98%, n = 5). E FACS and immunocytochemistry (ICC) staining revealed both subpopulations (ADSCgfp+ and ADSCgfp−) shared similar epitope markers for mesenchymal stem cells, but ADSCgfp+ subset showed significant upregulation of cardiac-related genes (n = 5 in each group). *p < 0.05; **p < 0.01

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