Functional tissue-engineered microtissue formed by self-aggregation of cells for peripheral nerve regeneration

Table 1 List of primer sequences used in this study

Target genes	Forward primer (5′–3′)	Reverse primer (5′–3′)	Annealing temperature (°C)
GAPDH	ATGGTGAAGGTCGGTGTGAACG	TTACTCCTTGGAGGCCATGTAG	55–62
BDNF	TGGAACTCGCAATGCCGAACTAC	TCCTTATGAACCGCCAGCCAATTC	57
VEGF	GGCTCACTTCCAGAAACACG	GTGCTCTTGCAGAATCTAGTGG	55
IL-4	CAAGGAACACCACGGAGAACGAG	CTTCAAGCACGGAGGTACATCACG	56
IL-10	CTGCTCTTACTGGCTGGAGTGAAG	TGGGTCTGGCTGACTGGGAAG	56
IL-13	CTCGCTTGCCTTGGTGGTCTTG	GCACAGGGAAGTCTTCTGGTCTTG	56

GAPDH glyceraldehyde 3-phosphate dehydrogenase, BDNF brain-derived neurotrophic factor, VEGF vascular endothelial growth factor, IL-4 interleukin-4, IL-10 interleukin-10, IL-13 interleukin-13

ISSN: 1757-6512