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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Puromycin-based purification of cells with high expression of the cytochrome P450 CYP3A4 gene from a patient with drug-induced liver injury (DILI)

Fig. 1

Immortalization of hepatocytes from iPSCs. A Scheme for generation of immortalized hepatocytes from iPSCs. Hepatocyte cells are induced from iPSCs (step 1). To immortalize hepatocyte cells, EpCAM-positive cells are sorted from iPSC-derived hepatocytes (step 2), and are infected with the lentiviral vectors carrying TERT, CDK4R24C, and cyclin D1 (step 3). B Phase-contrast micrograph of iPSC-K. iPSC-K cells on MEFs formed a flat and well-defined colony. C Phase-contrast micrograph of hepatocyte-like cells from iPSC-K. D Phase-contrast micrograph of immortalized iHep4 cells. E Immunocytochemistry of iHep4 cells using an antibody to CYP3A4. F Western blot analysis. Samples starting from left: K4DT (iHep4-E4-72 cells infected with CSII-CMV-hCDK4R24C, CSII-CMV-cyclin D1, CSII-CMV-hTERT), (iHep4-E4-74 cells infected with CSII-ALB-tetOff-ADV, CSII-TRE-Tight-cyclin D1, CSII-TRE-Tight-hCDK4R24C, CSII-CMV-hTERT), (iHep4-E8-50 cells infected with CSII-CMV-TetOff-ADV, CSII-TRE-Tight-cyclin D1, CSII-TRE-Tight-hCDK4R24C, CSII-CMV-hTERT) (Table 1)

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Stem Cell Research & Therapy

ISSN: 1757-6512