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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human MuStem cells repress T-cell proliferation and cytotoxicity through both paracrine and contact-dependent pathways

Fig. 2

Effect of human MuStem cells and bone marrow-derived mesenchymal stem cells on proliferation and phenotype of T-cells in peripheral blood mononucleated cells. a Representative profile (left panel) and quantification (right panel) of the proliferation of Cell Trace Violet (CTV)-labeled CD4+ and CD8+ T-cells in peripheral blood mononucleated cells (PBMCs) cultured alone or with unstimulated hMuStem cells (n = 5, independent batches) or bone marrow-derived mesenchymal stem cells (BM-MSCs; n = 4, independent batches). PBMCs were stimulated with phyto-hemagglutinin (PHA) to induce T-cell proliferation. The suppressive capacity of hMuStem cells or BM-MSCs was determined by tracking cell division of CTV-labeled PBMCs. b Representative FACS plots with gating of CD25+ cells in CD4+ and CD8+ T-cell fractions of PBMCs cultured alone or under PHA stimulation and with hMuStem cells or BM-MSCs. Percentages are expressed as the percentage of PBMCs cultured alone. c Representative FACS plots with gating of IL-10+, FoxP3+, IFN-γ+ and IL-17+ cells in CD4+ T-cell fractions of PBMCs cultured under PHA stimulation only and with hMuStem cells or BM-MSCs. Percentages are expressed as the percentage of PBMCs cultured alone. Data are presented as mean ± SEM (*p < 0.05, **p < 0.01, **p < 0.001, ****p < 0.0001; Mann–Whitney U test)

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