Fig. 5

Effect of human MuStem cells and human bone-marrow mesenchymal stem cells on the cytotoxic response of CD8⁺ T-lymphocytes. a Flow cytometry comparison of IFN-γ expression on CD8⁺ cytotoxic T-lymphocytes (CTLs) cultured either alone or with a MUC-1-expressing mesothelioma cell line. Native CTLs correspond to CTLs cultured in standard medium. Conditioned-CTLs correspond to CTLs co-cultured with hMuStem cells or BM-MSCs (unstimulated or TNF-α/IFN-γ-stimulated) or with their corresponding secretome (collected in both conditions). Percentage of IFN-γ⁺ CD8⁺ cells from one representative experiment are reported in the right upper corner of each corresponding plot. b Percentage of IFN-γ⁺ cells in CTLs co-cultured with hMuStem cells or BM-MSCs (unstimulated or TNF-α/IFN-γ-stimulated) or with their corresponding secretome. c Quantification of specific lysis of CTLs co-cultured with hMuStem cells or BM-MSCs (unstimulated or TNF-α/IFN-γ-stimulated) or with their corresponding secretome. d Quantification of granzyme B secretion by CTLs cultured with a MUC-1 expressing mesothelioma cell line. CTLs were cultured in basal conditions (native CTLs) or conditioned with either hMuStem cells and BM-MSCs (unstimulated or TNF-α/IFN-γ-stimulated) or their corresponding secretome. Experiments were performed on 5 and 4 independent batches of hMuStem cells and BM-MSCs, respectively. Data are presented as mean ± SEM (significant difference compared to native CTLs: ▴p < 0.05, ▴▴p < 0.01; significant difference between two conditions; *p < 0.05, **p < 0.01; Mann–Whitney U test or Wilcoxon matched-pairs signed rank test (unstimulated vs. stimulated condition)