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Table 1 Expression levels of immune cell-related markers measured in human MuStem cells

From: Human MuStem cells repress T-cell proliferation and cytotoxicity through both paracrine and contact-dependent pathways

Cell surface markers Unstimulated hMuStem cells TNF-α/IFN-γ-stimulated hMuStem cells
Positive cells (%) rMFI Positive cells (%) rMFI
Human leukocyte antigen
HLA-ABC 100% 13.3 ± 7.3 100% 21.2 ± 10.3
HLA-DP 29.2% ± 23.9% 9.7 ± 4.8 88.3% ± 23.5% 19.6 ± 10.5
HLA-E 0% NA 100% 4.0 ± 1.1
Immune-checkpoint ligands
Co-inhibitory factors
PDL-1 0% NA 100% 4.0 ± 1.3
PDL-2 100% 2.3 ± 0.3 100% 3.7 ± 1.1
Other factors
CD155 100% 12.0 ± 2.9 100% 14.0 ± 3.6
Adhesion molecules involved in T-cell interaction
ICAM-1 37.4% ± 11.2% 10.4 ± 6.0 100% 284.5 ± 124.8
VCAM-1 8.3% ± 4.0% 6.0 ± 3.7 100% 5.6 ± 3.2
  1. Flow cytometry comparison of human leukocyte antigen (HLA) molecules, immune-checkpoint ligands and adhesion molecules involved in T-cell interaction in hMuStem cells cultured in basal (unstimulated) or pro-inflammatory (TNF-α/IFN-γ-stimulation) conditions. Data are presented as the mean ± SEM (n = 5, independent batches) percentage of positive events for each marker and the relative mean fluorescence intensity (rMFI). NA, not applicable