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Table 1 Primer sequence of genes used in qRT-PCR analyses

From: Human amniotic mesenchymal stem cells combined with PPCNg facilitate injured endometrial regeneration

Gene

Forward primer sequence (5′-3′)

Reward primer sequence (5′-3′)

OCT4

GCAAGCCCTCATTTCACC

CCATCACCTCCACCACCT

SOX2

CGAACCATCTCTGTGGTCT

GTGTCAACCTGCATGGC

KLF4

AGGAGCCCAGCCAGAAA

TCCAGTCACAGACCCCATC

C-MYC

GCCAGAGGAGGAACGAG

GCTTGGACGGACAGGAT

CK7

TGGATGCCCTGAATGATGAGAT

GGGAGCGACTGTTGTCCA

CK8

CCATTAAGGATGCCAACGCCAA

TTCATCAGCTCCTGGTACTCAC

CK19

ACTACACGACCATCCAGGAC

GCAGAGCCTGTTCCGTCTCA

E-CDA

ACCATTCAGTACAACGACCCAA

GCCTTCCTACAGACGCCAG

ALP

GTACCATTTGCCCAGGTG

GGGCTCGCTCATCAAAG

RUNX2

GTAGATGGACCTCGGGAAC

TGCGCTACCTGAAACTGA

BSP

GGAGGAGACAATGGAGA

TTCAACGGTGGTGGTTTT

COL-II

GGATGGCTGCACGAAAC

CCCTATGTCCACACCGAAT

ACAN

TGCAGAACAGTGCCATCA

CTCCATAGCAGCCTTCCC

SOX9

AGCGCATTACCCACTTGT

ATCCTCCACGCTTGCTC

PPARγ

CGAGAAGGAGAAGCTGTTG

TCAGCGGGAAGGACTTTA

ADIP

TATCCCCAAGCCACACC

TCAGCCAGAGCAATGAGAT

LPL

TGCGTCTCTTTTGTTCCTG

CTCCATCTGGCTCATTGC

GAPDH

AACAGCCTCAAGATCATCAGC

ATGAGTCCTTCCACGATACCAA

  1. OCT4, octamer-binding transcription factor-4; SOX2, SRY-related high-mobility-group-box protein-2; KLF4, Kruppel-like factor-4; C-MYC, cellular-myelocytomatosis viral oncogene; CK-7, cytokeratin 7; CK-8, cytokeratin 8; CK-19, cytokeratin 19; E-CDA, E-Cadherin; ALP, alkaline phosphatase; RUNX2, runt-related transcription factor-2; BSP, bone sialoprotein; SOX9, SRY-BOX Transcription Factor 9; ACAN, aggrecan; COL-II, type II collagen; PPARγ peroxisome proliferator-activated receptor γ; LPL, lipoprotein lipase; ADIP, adiponectin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase
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Stem Cell Research & Therapy

ISSN: 1757-6512