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Fig. 3. | Stem Cell Research & Therapy

Fig. 3.

From: Thymosin beta-4 improves endothelial function and reparative potency of diabetic endothelial cells differentiated from patient induced pluripotent stem cells

Fig. 3.

600 ng/mL Tb4 increases proliferation and reduces senescence in dia-hiPSC-ECs. A dia-hiPSC-EC population doubling time. B Representative images of Western Blot analysis for protein expression of Cyclin D2 in dia-hiPSC-ECs. C Quantification of cyclin D2 protein expression. D Representative images of β-gal staining (green color) in dia-hiPSC-ECs. E Quantification of β-gal density in dia-hiPSC-ECs. Representative images of Western Blot for p21, p53, and acetylated P53 (Ace-P53) protein expressions in DP2-ECs (F) and DP3-ECs (G). Quantification of p21 (H), p53 (I), and Ace-P53 (J) protein expression in DP2-ECs and DP3-ECs. (Bar = 50 μm, n = 4). Values are presented as means ± SD. Independent T-test (**p < 0.01; ***p < 0.001)

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