Fig. 6

Tb4 improves dia-hiPSC-ECs viability against hypoxic injury in vitro. A The concentrations of lactate dehydrogenase (LDH) in the cell culture medium were measured in the absence or presence of Tb4 and presented as a percentage of the measurements obtained in the absence of Tb4. B The concentration of DNA fragments in the cell culture medium were measured in the absence or presence of 600 ng/mL Tb4. C Representative Western Blot images of dia-hiPSC-ECs cultured in EBM supplemented with or without 600 ng/mL Tb4 for protein expressions of pAKT, AKT, and Bcl-XL. Protein expression level of GAPDH was used as an internal control. Cells were cultured under hypoxic condition and were harvested at 0, 0.5, 1, and 3 h after treatment. D Quantification of pAKT protein expressions, which were expressed as percentages of AKT protein levels after normalized with GAPDH protein. E Quantification of Bcl-XL protein expressions, which were presented as percentages of GAPDH protein levels. F Pre-treatment with AKT inhibitor (AKTi) or Bcl-XL inhibitor (Bcl-XLi) blocked cytoprotective effect of Tb4. (Values are presented as the means ± SD. (A, B, F: n = 4, One-Way ANOVA; D&E: n = 3 for each sample and time point, independent T-test. *p < 0.05; **p < 0.01; ***p < 0.001)