Fig. 1

MSCs attenuate bleomycin-induced pulmonary fibrosis in mice. A H&E staining, Masson’s trichrome staining, Sirius red staining and representative immunofluorescence images obtained using an anti-α-SMA (green) antibody in lung sections from PBS-treated, Bleomycin-treated and Bleomycin + tdTomato⁺MSCs-treated mice (n = 6 per group). Scale bars: 50 µm. B Hydroxyproline levels in lungs of C57/BL6 mice from the different groups (n = 6 mice per group). C qPCR analysis of α-SMA, Col1a1 and Fn1 mRNA expression in lung slices from the different groups. D Western blot analysis of α-SMA and Collagen I in lungs from the different groups (n = 3 mice per group). E Quantification of α-SMA and Collagen I from D. Data are presented as the mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001; one-way ANOVA and Tukey’s multiple comparisons test