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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Mesenchymal stromal cells attenuate alveolar type 2 cells senescence through regulating NAMPT-mediated NAD metabolism

Fig. 5

MSCs inhibit AT2 cells senescence by suppressing lysosome-mediated NAMPT degradation. A Half-life analysis of NAMPT in primary AT2 cells co-cultured with MSCs. All cell groups were treated with cycloheximide (CHX, 50 μg/ml) harvested at the indicated times (0, 4, 8, 12 h after CHX treatment) and subjected to immunoblotting (n = 3 per group). B Quantification of NAMPT protein levels in A. C Immunofluorescence staining of primary AT2 cells co-cultured with MSCs and visualized using anti-LAMP1 (green) and anti-NAMPT (red) antibodies. Scale bars: 10 µm. D Western blot analysis of NAMPT expression in primary AT2 cells co-cultured with MSCs. E Immunofluorescence staining of primary AT2 cells co-cultured with MSCs and visualized using anti-NAMPT (red) antibodies. Scale bars: 10 µm. Data are presented as the mean ± SEM of three independent experiments

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