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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Mesenchymal stromal cells attenuate alveolar type 2 cells senescence through regulating NAMPT-mediated NAD metabolism

Fig. 6

NAMPT is required for MSCs inhibiting AT2 cells senescence. A SA-β-galactosidase staining of primary AT2 cells co-cultured with MSCs with or without FK866 treatment. B Immunofluorescence staining of primary AT2 cells co-cultured with MSCs and visualized using anti-P16 (red) antibodies. Scale bars: 10 µm. C qPCR analysis of P16 mRNA expression in primary AT2 cells co-cultured with MSCs with or without FK866 treatment. D qPCR analysis of P21 mRNA expression in primary AT2 cells co-cultured with MSCs with or without FK866 treatment. E Western blot analysis of P16 and P21 expression in primary AT2 cells co-cultured with MSCs with or without FK866 treatment. Data are presented as the mean ± SEM of three independent experiments; **P < 0.01, ***P < 0.001; one-way ANOVA and Tukey’s multiple comparisons test

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