Fig. 2
Cell morphology and mitochondrial volume evaluation by laser scanning confocal microscopy. A Representative fluorescence images of HCaMSC (a) and IPFaMSC (b). Scale bar = 20 μm. B. Representative images of mitochondria 3D isosurface in HCaMSC (a) and IPFaMSC (b) created by Imaris 8 software. Scale bar = 20 μm. C Statistical analysis of IPFaMSC and HCaMSC cells mitochondrial volumes was done by unpaired Student's t test. Dots represent individual cell values. No significant difference in the mitochondrial volume between two groups was found, with median values for IPFaMSC and HCaMSC of 596 and 702 μm3, respectively. Differences were considered significant when *p < 0.05, compared with control cell values