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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Optimization of human umbilical cord blood-derived mesenchymal stem cell isolation and culture methods in serum- and xeno-free conditions

Fig. 2

UCB-MSC characterization. a The morphology of CFU formed from cells cultured with StemMACS and the culture flask surface was coated with CELLStart and AutoSerum. b CFU efficacy in two coating conditions, CELLStart vs. AutoSerum. c Positive expression of MSC markers (CD90, CD73 and CD105) and negative markers (CD45, CD34, CD11b, CD19 and HLA-DR) from isolated UCB-MSCs. d RT-PCR revealed the expression of pluripotent markers of cMyc (328 bp), Nanog (285 bp) and Oct ¾ (144 bp), marker (M) 100 bp. e, f Trilineage differentiation of isolated UCB-MSCs. MSCs were cultured in osteogenic, adipogenic and chondrogenic induction media. Cells were used to performed qPCR (e) or stained with specific staining solutions (f) on day 14 and compared to undifferentiated control samples. g Karyotype analysis of UCB-MSCs showed no numerical or structural chromosome abnormalities. *p < 0.05; **p < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512