Table 1 Primer sequences
Gene | Lineage | Primer | Primer sequence |
|---|---|---|---|
ALP | Osteocyte | Forward primer | CCCGTGGCAACTCTATCTT |
ALP | Osteocyte | Reverse primer | AGTCCACCATGGAGACATTC |
PTH-R | Osteocyte | Forward primer | TGAACGGGAGGTGTTTGA |
PTH-R | Osteocyte | Reverse primer | GGTGCATGTGGATGTAGTTG |
COL I | Osteocyte | Forward primer | CCTGTCTGCTTCCTGTAAACTC |
COL I | Osteocyte | Reverse primer | GTTCAGTTTGGGTTGCTTGTC |
PPARg | Adipocyte | Forward primer | CCCTTCACTACTGTTGACTTCTC |
PPARg | Adipocyte | Reverse primer | TGCAGGCTCCACTTTGATT |
Leptin | Adipocyte | Forward primer | CCAGGATCAATGACATTTCACAC |
Leptin | Adipocyte | Reverse primer | CATACTGGTGAGGATCTGTTGG |
Sox9 | Chondrocyte | Forward primer | AACAAGCCGCACGTCAA |
Sox9 | Chondrocyte | Reverse primer | TCTCGCTCTCGTTCAGAAGT |
COL II | Osteocyte | Forward primer | ACACTCAAGTCCCTCAACAA |
COL II | Osteocyte | Reverse primer | GTCAATCCAGTAGTCTCCACTC |
GAPDH | Housekeeping genes | Forward primer | GGTGTGAACCATGAGAAGTATGA |
GAPDH | Housekeeping genes | Reverse primer | GAGTCCTTCCACGATACCAAAG |