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Table 3 The number and day that MSC-like cell colonies (MCC) appeared in different coating conditions

From: Optimization of human umbilical cord blood-derived mesenchymal stem cell isolation and culture methods in serum- and xeno-free conditions

Coating conditions

Isolated MCC per 108 MNCs

MCC detection (Day post MNC seeding)

(+)

(−)

(+)

(−)

Free

0.23 ± 0.32

0.27 ± 0.2

14.5 ± 5.4

17.2 ± 8.2

CELLStart

0.48 ± 0.78

0.41 ± 0.62

12.3 ± 3.2

11.4 ± 6.2

Nutristem Attach

0

0

0

0

AutoSerum

2.08 ± 2.50

1.8 ± 1.9

10.8 ± 5.8

11.9 ± 6.9

 

(+): with 10% AutoPlasma

(−): without 10% AutoPlasma