Fig. 1

Efficiency and protein kinetics of modRNA transfected ADSCs. A Representative images demonstrating GFP expression in GFP modRNA transfected ADSCs (the GFP-modRNA group) at 24 h after transfection (Scale bar = 100 μm) compared to ADSCs (the control group). B, C Transfection efficiency by flow cytometry analysis at 24 h after transfection. D Expression levels of IGF-1 mRNA in ADSCs, GFP-ADSCs and IGF-1-ADSCs at 24 h, 48 h and 72 h post-transfection. E Representative images demonstrating expression of IGF-1 protein in ADSCs, GFP-ADSCs and IGF-1-ADSCs at 16 h post-transfection. (Scale bar = 60 μm) F ELISA analysis of kinetics of IGF-1 protein from IGF-1 modRNA transfected mouse ADSCs (native ADSCs, the control group). G Western blot analysis of the type II collagen production of chondrocytes treated with the medium of ADSCs, recombinant IGF-1 protein or IGF-1 protein produced by IGF-1-ADSCs. (*P < 0.05, **P < 0.01, ***P < 0.001, ns not significant)