Fig. 2

Cytokine profiling within media conditioned by cell products. A Proteomic profiling showing differentially expressed cytokines within media conditioned by cell products. The 50th and 90th percentile marks for mean pixel density are indicated to visualize cytokine abundance. Further, Venn diagram was used to show the number of cytokines that are distinct or common among cell types. Both HDCs and UC-MSCs showed robust cytokine production in comparison to BM-MSCs. Data were compared to baseline using Two-way ANOVA testing followed by with post hoc testing using Dunnet’s Multiple Comparisons test to determine the group(s) with the difference(s). *p < 0.05 compared to baseline. All data are presented as individual and mean values ± SEM, n = 3 biological replicates; each circle represents one data point from one unique biological replicate. B Volcano plots demonstrating the relationship between difference in cytokine expression by each cell type and the False Discovery Rate (i.e., the q value). HDCs produced 37 more cytokines vs. BM-MSCs (only 1 cytokine found to be greater in BM-MSCs). UC-MSCs produced 19 more cytokines vs. BM-MSCs and UC-MSCs produced 10 cytokines in greater abundance vs. HDCs (only 2 cytokines in greater abundance in HDCs). Data were compared using an unpaired t-test with individual variances for samples and a two-stage step-up (Benjamini, Krieger, and Yekutieli) false discovery rate to account for multiple comparisons